Complementary Techniques Assisted Reproduction
Micromanipulation techniques in assisted reproduction are complementary techniques to in vitro fertilization (IVF), indicated in special cases to improve outcomes. Several types exist, including assisted hatching, embryo makeup, and embryo biopsy.
Embryonic Makeup is one of the new Assisted Reproductive Technology techniques that allows the removal of cell fragments that hinder embryo development.
Human embryos contain membrane-bound fragments of cytoplasm between the embryo's cells. These originate during embryonic division and impede subsequent development. Embryonic Makeup involves creating a small opening in the zona pellucida and aspirating as many of these fragments as possible.
Embryo biopsy is performed to conduct a genetic study of the embryo (Preimplantation Genetic Testing). It involves extracting one of its cells for analysis. This can be done either on the third day of development or between the fifth and sixth day, at the blastocyst stage. It is a highly precise technique that does not alter the embryo's development.
The sample obtained through biopsy is processed and subjected to genetic analysis using Next Generation Sequencing (NGS).
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